Toxicologia dos óleos essenciais de citronela (Cymbopogon sp) e guaçatonga (Casearia sylvestris Swartz) em embrião de galinha (Gallus gallus domesticus Linnaeus)

Abstract

The present work sought to analyze the effects of Citronella (Cymbopogon sp) and Guaçatonga (Casearia sylvestris) essential oils comparing with effects of the iprodione-based product on the embryonic development of Gallus gallusdomesticus. The experiments were carried out at the Biological Control Laboratory II at Universidad Technological Federal do Paraná – UTFPR, DV. Each experimental group (containing forty eggs) received the following concentrations: vehicle control group (1) received 100µl of 80 to 1% Tween® in distilled water and the vehicle control group (2) received 100µl of 80 to 1% Tween® in saline, the groups with essential oils of Citronella and Guaçatonga received 100µl, being 62,5µg/ml and 4.000 µg/ml, respectively, in distilled water. Lastly, the group containing synthetic phytosanitary product, the eggs were injected with 100µl of Iprodione-based fungicide in distilled water (at the recommended dose by the manufacture). Such doses were injected into the egg air chamber and were incubated for three days. After incubation, the embryos were collected and analyzed. Among the treatments, the EOs and the phytosanitary product presented toxicity to the embryos development, however, the product caused more damaged to the embryos. The closed control group presented results within the expected. Both treatments with Tween® 80 caused deleterious effects on the development; interestingly, the Tween® with lower concentration caused more harmful effects. Such results alert us about the effects EOs can cause, as week as the phytosanitary product and interestingly, the Tween®80. These data seek to contribute to the safety knowledge of important control agents for alternative production systems, aiming at the use of natural and safe products to the environment and human health.