Gestão da inovação agroindustrial: diagnóstico molecular

Abstract

The technique PolymeraseChain Reaction (PCR) is a techniquethatallows the amplification of regions of the genome from small amounts of Deoxyribonucleic Acid (DNA) in vitro, it is the basis of many techniques of molecular biology modern, this technique was started more than a century ago by several researchers. The first visualization of the DNA molecule was performed in 1848 by the German botanist Wilhelm Hofmeister. Real-time PCR is one of the techniques considered to be the most accurate for quantify levels of expression of a particular gene and it is also possible to obtain results fast and reproducible. In this way, the PCR technique has shown applications in several areas that require rapid diagnosis and still allows results are obtained from small amounts of DNA. Molecular diagnosis PCR has been an important technological innovation since the 1980s, where a revolution in molecular genetics due to the new approach to gene research; thus it can be said, therefore, that the great advances in molecular times, have allowed improvements, mainly in the agroindustrial sector, specifically in the food industry, with the rapid development of new technologies which is generating a wealth of data, important for more detailed taxonomic assessments.