Análise do potencial biotecnológico do extrato de propolis vermelha como alternativa no tratamento de lesões por pressão

Abstract

Red propolis is widely recognized for its antimicrobial properties across various studies, although its effectiveness against different bacterial strains has yet to be fully elucidated. This study aims to investigate the potential of red propolis extract by evaluating its biological properties and possible biotechnological applications as a therapeutic alternative for the treatment of pressure ulcers. Furthermore, it seeks to determine the efficacy of this extract in inhibiting strains of Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, and Proteus mirabilis, with the goal of facilitating its biotechnological use in the treatment of patients with pressure injuries. Red propolis extracts were prepared in various concentrations to conduct antimicrobial activity tests, using the microdilution method to identify the Minimum Inhibitory Concentration and Minimum Bactericidal Concentration in relation to ATCC bacterial strains. These extracts were also used to evaluate cytotoxic activity, employing Artemia salina Leach. Additionally, studies were conducted to determine the antioxidant activity of the extracts through DPPH and ABTS analysis, as well as to assess anti-inflammatory activity via MTT analysis and wound-healing capacity through cellular migration analysis. The results of this study highlight red propolis as a promising source of bioactive compounds with significant antibacterial and antioxidant activity. Although the anti-inflammatory response was not pronounced, the high cell viability and potent antioxidant activity suggest that red propolis has considerable potential for the development of new therapeutic agents. Further studies are necessary to fully explore this potential and to optimize extraction methods to enhance its immunomodulatory properties.