Caracterização de isolado produtor de nanocelulose bacteriana a partir de vinagre de maçã

Abstract

Bacterial nanocellulose (NCB) is an environmentally friendly biopolymer with several commercial applications. Its use is currently limited by the capacity to produce bacterial strains and the cost of culture media. The search for new organisms with less complex growth conditions is important for the adoption of NBC. This biopolymer presents itself as an extracellular polysaccharide, secreted by some species of bacteria, in addition to some algae and tunicates, it has interesting properties such as biocompatibility, high purity, biodegradability, fiber resistance and high-water retention, and can be applied in several sectors such as health, food, electronics, cosmetics, textiles, among others. Its use is currently limited by the productivity capacity of bacterial strains and the high cost of growing media. The search for new microorganisms with less complex growth conditions is important for the adoption of NBC. In this study, a new nanocellulose-producing strain was isolated from organic apple cider vinegar and identified by sequencing 16S rARN gene. The genera Sphingobium and Sphingomonas were the most representative of the analysis, obtaining a value above 98% of identity. At the species level, Sphingobium yanoikuyae and Sphingomonas paucimobilis were the most representative with 98.65% and 98.83% of identity, respectively. All membranes obtained, both by conventional culture and alternative culture medium were characterized by light microscopy. In addition, morphological characterization of the consortium of bacteria present in the vinegar, the isolate producing NCB and the standard strain K. hansenii was performed.