Formulação de meio de cultivo para pseudomonas fluorescens

Abstract

The adoption of an ecofriendly posture is an alternative for the development of an agriculture with less environmental impact. The use of rhizospheric microorganisms has been shown to be an excellent alternative for organomineral fertilization. Among these microorganisms Pseudomonas fluorescens presents itself as an excellent solubilizer of phosphate present in the soil, avoiding the need to apply nitrogen and phosphate fertilizers, which, although they help in the development of the plant, much of it remains in a residual form in the soil, being able to calcify and destabilize. the pH of the soil, so the application of P. fluorescens is positive both in reducing the application of fertilizers and in the synthesis of residual compounds. The species is characterized as a Gram-negative bacillus, producer of fluorescent pigments, capable of producing biofilms as well as degrading various compounds in the presence or absence of O2. Knowing the morphological aspects of this microorganism is essential for its production on an industrial scale and the development of a quality control system. The objective of this study was to formulate a specific culture medium for the production of P. fluorescens on an industrial scale. The analyzes were carried out at the Fertibio do Brasil inoculant industry, where colony and cell morphology analyzes were carried out, biochemical profile definition through 15 tests adapted from the literature, formulation of specific culture media, standardization of fermentative parameters and cost analysis. Through the morphological analysis, it was confirmed that the strain, P. fluorescens ATCC 13525 as a Gram-negative bacillus, capable of carrying out the synthesis of carbohydrates, with oxidative potential, nitrate reduction, and facultative aerobics with the definition of biochemical profile. three culture media were formulated, which underwent analysis of cell concentration and stability, in order to define the most suitable for the microorganism in question, all data obtained were statistically evaluated using the ANOVA and Tukey test, at the end of the study if established medium C as the most suitable for the production of inoculant based on P. fluorescens, as it reached the minimum cell concentrations, in addition to offering adequate support for quality control, and the parameters of temperature fermentations were also defined. and stirring at 30°C and 100rpm respectively.