Antissépticos comerciais utilizados em vacas leiteiras: susceptibilidade in vitro frente a bactérias e leveduras

Abstract

Brazil is among the largest dairy producers in the world. In order to produce and market good quality milk, hygienic-sanitary measures are necessary, including to combat mastitis, causing economic losses to the producer and, consequently, reducing production. The objective of this study was to evaluate the effect of two commercial antiseptics used on the ceilings of dairy cows before and after milking, in order to verify their antimicrobial activity on strains of Echerichia coli, Staphylococcus aureus and Candida albicans. The methodological procedures were carried out in the laboratory of Microbiology of the Federal University of Technologic-Paraná, Dois Vizinhos, by means of pH and disc diffusion and microdilution techniques in triplicate in order to obtain the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC).The pH of the products deviated from that described on the label and could compromise their efficacy. In the disc-diffusion test, E. coli presented intermediate sensitivity, whereas the other microorganisms were sensitive to the products pre- and post-milking. In the microdilution tests in broth, the microorganisms showed sensitivity to the pre and post-milking products, allowing to verify that they presented MICs and CBM even diluted, being verified that the combinations of the components used in the formulations destroy the main etiological agents of mastitis, although not dilution is recommended for use.