Análise citogenética de Ancistrus Kner, 1854 da região sul do Brasil

Abstract

The objective of this work was to perform cytogenetic analysis in fish species belonging to the genus Ancistrus. The collection was held in Águas Mornas, Cubatão River basin and in the town of Santa Helena, in the basin of the Paraná River 3. Kidneys were dissociated in potassium chloride solution and placed in a fixation solution. Chromosomes were treated with the Giemsa staining protocol, silver precipitation, C banding and FISH. The karyotype formula was 29m. + sm. + 23a. + t. in 3 females, 22m. + sm. + 27a. + t. on 2 females, 21m. + sm. 30a. + t. in one male and one female and 28m. + sm. 22a. + t. in 3 males and 2 females The AgRONs were found near the centromere in a pair of acrocentric chromosomes in fish of Saint Helena, in fish collected in the Rio Cubatão to AgRON appeared in a single acrocentric chromosome. The regions rich in heterochromatin were in most telomeric regions and centromeric chromosomes in all fish. The genus Ancistrus possess a wide variation in the number and structure of the constitutive heterochromatin diploid. Nevertheless, the relatively constant AgRONs.