Aumento do teor proteico da biomassa seca da cianobactéria Spirulina platensis por extração de carboidratos utilizando solvente

Abstract

Spirulina is widely used in dietary supplements because it has a high nutritional content and also because it has an enormous availability of nutrients with a percentage of 50% - 70% of proteins. The objective of this study is to increase the protein content of dry Spirulina platensis biomass, and it is necessary to understand the effect of solvent application on the extraction of carbohydrates from biomass through a chemical treatment proposed by an advanced statistical tool. The exploratory research method was applied through a Plackett & Burman factorial matrix, in order to optimize the parameters of solvent concentration (in a percentage of 10, 50 and 100%), biomass concentration (in 1, 5,5 and 10g/L), extraction temperature (in 80, 100 and 120°C) and extraction time (in 5, 15 and 25min). Above the parameters, significant and correlated responses were obtained for solvent concentration factors, biomass concentration factors and temperature. With the best conditions of carbohydrate and proteins extraction through the chemical treatment, the results for the experiments of numbers 6 and 10, respectively, are: 10g/L of biomass, 10% of ethanol, 120°C of temperature and 25 minutes of extraction time and 1g/L of biomass, 10% of ethanol, 80°C of temperature and 5 minutes of extraction time.