Otimização do método de doseamento de secnidazol via DOE

Abstract

The present work is of paramount importance for the optimization of drug analysis methods, as, with the application of new work tools, such as the Design of Experimens (DOE), it will be able to increasingly evolve the quality of projects, and, parallel to this, decrease the repeatability of the analysis of new and currently used products. This work will be developed based on an existing method of secnidazole. Aiming at optimizing the raw material secnidazole dosing method that is currently validated and used. For the development of this project, the technique of liquid chromatography and mathematical statistical analysis was used as the main means of data processing. Therefore, after testing the current method in a high-performance chromatograph (HPLC), it was transferred to an ultra-performance chromatograph (UPLC), complying with the safety specification of the equipment in question. The previously acquired results were used to provide a theoretical and assertive foundation for the proposed test, so that the objective is achieved with greater efficiency and quality. After acquiring the desired data in the work, analyzes were carried out to ensure the repeatability and robustness of the method developed in the work so that it can be reproduced by the industry. At the end, it can be seen that the application of the DOE methodology was suitable for the analysis of secnidzole, using UPLC.