Diferentes concentrações de manipueira para desenvolvimento de Trichoderma spp. em meio submerso a partir de um biorreator airlift alternativo

Abstract

The starch manufacturers are processing of manioc industries to obtain starch. There are several units installed in Paraná State and northwest middle region containing higher concentration. This scenario contributes to the generation of a large amount of polluting residual waters, especially manipueira. The study aims to evaluate the effectiveness of cassava as alternative culture in different concentrations using Trichoderma spp. grown in an alternative airlift bioreactor. The manipueira was collected of a cassava industry in the municipality of Araruna-PR. With this set up three different concentrations of the effluent as a substrate for cultivation of Trichoderma spp. This fungus has been granted by a company that produces for commercial purposes and may find the presence of 1,1x1011 UFC.mL-1. Firstly it must prepared the pre inoculum of 400 mL, 10 mL which is the fungus Trichoderma spp. and the remainder of the proposed substrates. After 72 hours in shaker table, the pre inoculum can be introduced in alternative airlift bioreactor working volume of 5 liters. Again, after 72 hours in the bioreactor this product generated will be removed. The bioreactor produce PVC material, height 70 cm, with a supercharger and diffuser both of aquarium for air spraying. Already the temperature, it was under the effect of a cartridge resistance 100 watts. The three different concentrations were tested, 50 mL.L-1; 125 mL.L-1 and 175 mL.L-1, of cassava as a substrate, and through comparative tests and testing colony forming units, was able to establish the presence of the 1x106 1,2x109 UFC.mL-1 the alternative airlift bioreactor and then evaluate them. With a pH range between 5.6 the 4.8. Dissolved oxygen showed a range from 6.4 to 0.55 mg L-1. Since the temperature was controlled by a variable resistor of a cartridge, which remained around 28±1. There was no difference in growth between the concentrations, so we recommend the use of 175 mL.L-1 concentration due to manipueira be an environmental liability.