Extração e caracterização do brodifacoum em raticida comercial

Abstract

Many pieces and crumbles of wheat-based commercial rodenticides arrive at Paraná’s criminal institute laboratory being extracted from liquids such as coffee and tea. Therefore, it is essential the use of analysis methods to determine the existence of their active ingredients, mainly consisted of coumarin derivatives, in forensic investigations; however, the products sold contain low levels of these compounds which makes the analysis difficult. The objective of this report was to develop an extraction method capable to extract the brodifacoum coumarin from commercial samples and determine it by high-performance liquid chromatography (HPLC) with a diode array detector (DAD). The extraction was done at Universidade Tecnológica Federal do Paraná (UTFPR) Ecoville campus and the rest of the analysis was done at the city medical examiner's office laboratory. A brodifacoum determination method was introduced to analyze wheat-based commercial rodenticide that requires samples of less than 10 mg. The method of extraction involves sonicating the sample and analyzing by HPLC-DAD. Through this method, it was possible to extract the compound without an extensive sample clean up. The estimated limits of detection (LOD) and quantification (LOQ) were 0,153 and 0,510 mg L-1 respectively and the recoveries were 51, 61 and 62% for three different commercial brands.